Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Polymerase Chain Reaction (PCR)
Primers and Probes
Oligo d(T)18mRNA Primer
Supplier Images
Oligo d(T)18mRNA Primer
Oligo d(T)18mRNA Primer from New England Biolabs
Be the first to write a review!
Citations:
(10)
Supplier Page
Supplier Page from
New England Biolabs for
Oligo d(T)18mRNA Primer
Get Pricing
Description
This primer is used for the priming and sequencing of mRNA adjacent to the 3-poly A tail (1).
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Oligo d(T)18mRNA Primer
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
S1316S
Target
Oligo d(T)18 (no 5'-Phosphate)
Quantity
5 a260 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Primer Type
Oligo dT Primers
Type
Oligo
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (10)
(1)
Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism
Communications biology
January 4, 2023
Landowski M, Bhute VJ, Grindel S, Haugstad Z, Gyening YK, Tytanic M, Brush RS, Moyer LJ, Nelson DW, Davis CR,
et al
.
Scientific, Waltham, MA). One microgram of RNA was used to make cDNA using an Oligo d(T)18 primer (#
S1316S
,
NEB
, Ipswich, MA) and the
ProtoScript II Reverse Transcriptase
(#
M0368L
,
NEB
, Ipswich, MA) following
(2)
A mutation in transmembrane protein 135 impairs lipid metabolism in mouse eyecups
Scientific reports
January 14, 2022
Landowski M, Bhute VJ, Takimoto T, Grindel S, Shahi PK, Pattnaik BR, Ikeda S, Ikeda A.
Scientific, Waltham, MA). Equal RNA concentrations were aliquoted to make cDNA using an Oligo d(T)18 primer (#
S1316S
,
NEB
, Ipswich, MA) and the
ProtoScript II Reverse Transcriptase
(#
M0368L
,
NEB
, Ipswich, MA). Triplicate
(3)
Inactivation of AUF1 in Myeloid Cells Protects From Allergic Airway and Tumor Infiltration and Impairs the Adenosine-Induced Polarization of Pro-Angiogenic Macrophages
Frontiers in immunology
January 1, 2022
Gargani S, Lourou N, Arapatzi C, Tzanos D, Saridaki M, Dushku E, Chatzimike M, Sidiropoulos ND, Andreadou M, Ntafis V,
et al
.
cDNA synthesis
0.5-2μg of total RNA was reverse transcribed using MMLV-RT (
Promega
,
M1705
) and Oligo dT (
NEB
,
S1316S
) at 37°C for 1h.
qRT-PCR
was performed using EvaGreen SsoFast mix (
Bio-Rad
, Hercules, CA, USA,
172-5201
)
(4)
Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors
Cancers
October 13, 2021
Azatyan A, Zhang S, Darabi A, Siesjö P, Wang T, Zaphiropoulos PG.
performed as described above. Moreover,
cDNA synthesis
was also performed using oligo(dT) primers (
S1316S
,
NEB
, Ipswich, MA, USA). In the reverse transcription reaction, while random hexamers prime both linear
(5)
Head and neck squamous cell carcinoma cell lines have an immunomodulatory effect on macrophages independent of hypoxia and toll-like receptor 9
BMC cancer
September 3, 2021
Ishizu T, Eichin D, Padzik A, Tuominen S, Grénman R, Salmi M, Grönroos TJ, Tuomela JM.
RiboLock RNase inhibitor (
Thermo Scientific
, #
EO0381
) and the oligo-dT mRNA primer (
New England BioLabs
, #
S1316S
). 100 ng of cDNA from the
in vitro
samples was amplified with DyNAmo HS SYBR Green (
Thermo Scientific
,
(6)
SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt
Developmental cell
April 19, 2021
Burckhardt CJ, Minna JD, Danuser G.
using SuperScript IV (
Thermo Scientific
,
18091050
). In brief, 1 μg mRNA, oligo-d(T)18 primer (
NEB
,
S1316S
) and dNTPs (Bio Basic) were heated to 65 °C for 5 minutes and annealed on ice for three minutes. SuperScript
(7)
Modulation of Tmem135 Leads to Retinal Pigmented Epithelium Pathologies in Mice
Investigative ophthalmology & visual science
October 1, 2020
Landowski M, Grindel S, Shahi PK, Johnson A, Western D, Race A, Shi F, Benson J, Gao M, Santoirre E,
et al
.
Scientific, Waltham, MA, USA). Equal RNA concentrations were used to make cDNA using an Oligo d(T)18 primer (#
S1316S
;
NEB
, Ipswich, MA, USA) and the
ProtoScript II Reverse Transcriptase
(#
M0368L
;
NEB
) following the manufacturer's
(8)
Innate Sensing through Mesenchymal TLR4/MyD88 Signals Promotes Spontaneous Intestinal Tumorigenesis
Cell reports
January 15, 2019
Koliaraki V, Chalkidi N, Henriques A, Tzaferis C, Polykratis A, Waisman A, Muller W, Hackam DJ, Pasparakis M, Kollias G.
Transcriptase,
Promega
,
M1701
Platinum SYBR-Green
qPCR
SuperMix,
Invitrogen
,
11733046
Oligo-dT primers,
New England BioLabs
,
S1316S
Azoxymethane (AOM),Sigma-Aldrich,A5486 Dextran Sodium Sulfate (DSS),MP Biomedicals,M9147 LPS from
(9)
Translational Reprogramming Provides a Blueprint for Cellular Adaptation
Cell Chemical Biology
November 1, 2018
Max Berman Ferretti, Jennifer Louise Barre, Katrin Karbstein
DNase,
Promega
,CAT#
M6101
ProtoScriptII,
New England Biolabs
,CAT#
M0368S
Oligo dT,
New England Biolabs
,CAT#
S1316S
Excella™ SYBR MasterMix IQ,Worldwide Medical Products,CAT# 61071103
Experimental Models
: Cell Lines,
Experimental Models
(10)
Developmentally regulated alternative splicing is perturbed in type 1 diabetic skeletal muscle
Muscle & Nerve
October 1, 2017
Curtis A. Nutter, Elizabeth Jaworski, Sunil K. Verma, Yareli Perez‐Carrasco, Muge N. Kuyumcu‐Martinez
as described elsewhere.27,34 Briefly, a cDNA library was generated by annealing 125 ng of oligo(dT) (
S1316S
;
New England BioLabs
, Ipswich, Massachusetts) to the poly(A) tail of total RNA (1 μg) at 65° C for 10
<<
<
>
>>
Reviews
Be the First to Write a Review!
Oligo d(T)18mRNA Primer from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top